Humulin 50/50
R/Neutral/NPH/Isophane Mix by Eli Lilly
intermediate-acting r-DNA/GE/GM
U100 isophane
Line Humulin
Also known as Humulin M5
Similar to  
Action in cats  
  • onset Xh,
  • peak Xh
  • duration Xh
Action in dogs  
  • onset 0.5-3h,
  • peak 2-10h,
  • duration 4-24h
Use and Handling
Type cloudy
Shelf Life 24 months
When opened 28 days room temp.
In pen 28 days room temp.

Humulin 50/50 is the US brand name for Lilly's r-DNA/GE/GM human insulin mix consisting of 50% R/neutral insulin and 50% NPH/isophane crystal[2] insulin[3].

It is the same as Mixtard 50[4][5], Actraphane 50/50[6][7] and Aventis' Insuman Comb 50[8][9].

Lilly stopped offering this insulin, under the brand name Humulin M5[10], in the EU & UK in July 2004[11][12].


  1. Medsafe NZ Penmix 50
  2. EMEA Scientific Discussion--Mixtard
  3. Humulin 50/50-NPH;R Human Insulin
  4. Electronic Medicines Compendium (EMC) UK-Patient Information-Mixtard 50
  5. Insulins & Mixtures
  6. EMEA Scientific Discussion--Actraphane
  7. Prescribing Novo Insulins
  8. Electronic Medicines Compendium (EMC) UK Patient Information Leaflet-Insuman Comb 50
  9. Prescribing Insuman Insulins
  10. Lilly EU/UK Product Sheet & Time Activity Profiles
  11. Withdrawal of Humulin M5 July 2004
  12. Lilly EU/UK Product Names & Time Activity Profiles